ΑΝΑΛΥΣΗ ΑΙΘΕΡΙΩΝ ΕΛΑΙΩΝ ΑΡΩΜΑΤΙΚΩΝ ΚΑΙ ΦΑΡΜΑΚΕΥΤΙΚΩΝ ΦΥΤΩΝ - ΜΕΛΕΤΗ ΑΝΤΙΟΞΕΙ∆ΩΤΙΚΗΣ ΚΑΙ ΑΝΤΙΦΛΕΓΜΟΝΩ∆ΟΥΣ ∆ΡΑΣΗΣ ΤΩΝ ΦΥΤΩΝ

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Διαμάντω Λάζαρη

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<ul><li> 1. - </li></ul> <p> 2. . : , . 3. A : , . , . ( ). 4. : . , . , . 5. ( ) . . . 6. : / . , ( ). : . 2 - 95 %, . : . . 7 Thymus vulgaris. 7. : , Melissa (), Origanum (), Salvia (), Sideritis ( ) Thymus (), . . : - (, , , , ) . . - . - . - . - . 8. Thymus spp. (Lamiaceae) . ( ) . 9. Thymus spp. (Lamiaceae) . . , . (,), . . , , . . 10. Melissa officinalis (Lamiaceae) (, , , ). . , , . 11. Melissa officinalis (Lamiaceae) , , , -, , . , , , , , , , . 12. Melissa officinalis (Lamiaceae) . , . . . 13. (Origanum spp.) Origanum. . 14 . Origanum vulgare subsp. hirtum. 14. Origanum vulgare (Lamiaceae) - , , , ... . , , , , . , , . , , , . . 15. Origanum vulgare (Lamiaceae) () . . , . . 16. (Sideritis spp.) - Lamiaceae 17. Sideritis (, ) () ... - AIDS Melofer Sideritis scardica, 200 ppm 18. Salvia officinalis (Lamiaceae) () , , , , , , , , (, - , , .). , , , . 19. Salvia officinalis (Lamiaceae) () (, , ) ( ), , , ( ), . . , . 20. , . , , , 21. . , DNA. .. . . (LP) . 22. , . : , , . , , , Alzheimer . 23. SOD, , - - : , C, -, - ( Q). , . 24. , . 25. , , (, ), , ( ), , . 26. Clevenger, (Hellenic Pharmacopoeia, Ed. IV, Vol. I. Athens, 1989). . , 2 ml (GC grade) . , - 4C, . Hellenic Pharmacopoeia, Ed. IV, Vol. I. Athens, 1989: 191-193. 27. (% / ) MO-M0,1MO-Ml0,71 MO-Al 0,77(% / )MO-Ol0,82 OV-M3,97 OV-A5,26 OV-O4,45 SF-M2,39 MO: Melissa officinalis SF-A2,41 OV: Origanum vulgare subsp. hirtumSF-O2,55 SF: Salvia fruticosa SSFP-M 0,23SS: Sideritis scardica SSFO-M 0,16SSLP-M 0,25 TL: Thymus longicaulisSSLO-M 0,12 TS: Thymus sibthorpii TL-M2,92 TL-A2,19 TL-O2,97 TS-M2,09 TS-A2,84 TS-O2,58 28. - - , (GC-MS). GC-MS , SHIMADZU GC-2010 GCMS-QP2010 (70 eV). : 2300C, split ratio 1/30. HP-5MS (30 m x 0.25 mm, film thickness: 0.25 m). : 50 C (5 min) 290 C, 4 C/min. (1.0 ml/min). 1 l. (total ion count) , . (Rt) , NIST21 NIST107 (Massada, 1976) (Adams, 2001), RI (retention indices) Van den Dool &amp; Kratz (1963), (C9-C23). , . Adams, R., 2001. Identification of Essential Oil Components by Gas Chromatography/ Mass Spectroscopy. Carol Stream, Allured Publishing Co, Illinois. Massada, Y., 1976. Analysis of essential oil by gas chromatography and spectrometry. John Wiley &amp; Sons, New York. Van den Dool, H., Kratz, P. D., 1963. Journal of Chromatography 11, 463-471. 29. (x1,000,000)TIC 1.751.501.251.000.750.500.25 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 MO-M 30. (x10,000,000) 3.00 TIC2.752.502.252.001.751.501.251.000.750.500.250.005.010.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 OV-M 31. (x10,000,000) 5.0 TIC 4.5 4.0 3.5 3.0 2.5 2.0 1.5 1.0 0.55.010.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0SF-M 32. (x1,000,000) TIC 1.0 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.15.0 10.0 15.0 20.0 25.030.0 35.0 40.0 45.0 50.0 55.0 60.0SSLP-M 33. (x10,000,000)TIC 2.752.502.252.001.751.501.251.000.750.500.250.00 5.010.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 TL-M 34. (x1,000,000) TIC 5.0 4.5 4.0 3.5 3.0 2.5 2.0 1.5 1.0 0.5 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 TS-M 35. , 1,1 2 (DPPH), , . 36. 1,12 (DPPH) () DPPH (0.1 M) , (20 l DMSO 200 l DMSO). 60 min 517nm. O2NNO2. NN NO2 DPPHRHe,H+-R .O2NNO2NHN NO2 37. In vitro , . pH 7.4, . (50 ), (0.5 mM), (0.4 mM) ( 0.1 mM) 7.4 (50 mM), 10 37C . 40% 2%. 90C 20 . , 535 nm. 38. (LOX) DMSO (50 mg 2 ml DMSO). 100 l DMSO (0.1 mM) 0.2 ml (1/3x10-4, w/v ) pH=9.0 (Tris). 13-- 234 nm . 39. 120 100 80 % DPPH 10l/ml 60AAPH % 10l/ml LOX % Inh. 10l/ml 40 200-O-M -MO-M -Ml -O -AM-A-M l l -M -M-MA -A -O-M V-V- V-SF TSTSSF OLO TSTLSF O FPLP FO OOO O OM MM M SS SSSS SS DMSO 4mg/ml 40. 90 80 70 60 50 % DPPH 10l/ml AAPH % 10l/ml40 LOX % Inh. 10l/ml30 20 100-O -M-M O -M -MM l-ASS -OM-A SS - Ml M l -M SS -MSS - M A-O -A-M V-V- V-SFTS TS OSF LO TL TS SF O FPFOLP OO OO OMM 41. </p>