Η ΦΩΤΟΝΙΚΗ ΣΤΗΝ ΥΠΗΡΕΣΙΑ ΤΗΣ ΒΙΟΤΕΧΝΟΛΟΓΙΑΣ Νανοσωματίδια στην διάγνωση του καρκίνου

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. .. : . . . QD - PowerPoint PPT Presentation

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  • ..: .

  • QD ( - ) , ( ) Stokes shift

  • Fluorescence lifetime ( ) ( 0.5-20ns)Fluorescence quantum yield ( )

  • QDs Bulk Semiconductors Quantum Dots ( )

  • QDs Bulk Semiconductors

  • QDs Bulk SemiconductorsQuantum Dots QDs 100 1000 10 50 2 10 nm.To BER (Exciton Radius, Bohr)

  • QDs Bulk Semiconductors QDs quantum confinement effect ( ), QD

  • QDs Bulk Semiconductors QDs -> -> QDs -> -> QDs (400-4000nm)

  • (dyes) . QDs

  • T QDs , . Molar extinction coefficient- ) (0.5-2x10^6 /M cm QDs) (5-10x10^4/ M cm -organic dyes) () 10 20 (), (photobleaching), Multicolor QDs

  • (photobleaching)

  • Multiplexing of QDs signals( ) o ( , 25-30 nm full width at half maximum). .-> , . .

  • utofluorescent ( ) . QDs . QDs -. .

  • utofluorescent ()

  • VI (.. CdSe ,CdTe) V (.. InP ,InAs) . QD CdSe (core) ZnS (shell) . (ligand) TOPO (tri-n-octylphosphine oxide) , (ligands) .

  • :) (ligand) -> . ) ( ) , (polyethylene glycol, PEG) QD (water soluble) (absorption spectra, emission spectra, fluorescence quantum yields)

  • polymer-coated QDs (affinity ligands)

  • QDs

  • In vitro = , In vivo =

  • In vitro

  • ELISA (enzyme-linked immunosorbent assay)->>, , , QDs , , , , photobleaching () ->

  • QD ( )

  • QD () (nonfluorescence dye, )->>FRET (Fluorescence resonance energy transfer) (60% ) QD

  • FRET (Fluorescence resonance energy transfer, )H () - (donor-acceptor), .r= - (donor-acceptor), Ro 50% 'D D fluorescence lifetimes ( )

  • ..

  • DNA (capture probe reporter probe) QD 488nm 605 nm QD FRET acceptor reporter dye

  • QDs (microbeads) ->> 6 QDs (m) 10 QDs (n) 1 (n^m-1 ) .

  • microbead (20 ) DNA ( DNA ), microbeadO DNA target signal ( ).

  • In vivo Ultrasound CT, X-Ray CT, PET (SPECT), MRI , () () 200-400nm, - (deep-tissue optical imaging) (- )

  • In vivo Rayleigh (Rayleigh cross section ^4 (~^-4) (photobleaching effect) QDs (R)

  • QDs triblock copolymer () in vivo , (ligands) PEG (polyethylene glycol) ( ) QDs

  • :T Angiogenic tumors->> . : PSMA (prostate specific membrane antigen) H QDs

  • QDs Two-photon excitation ( ) QDs NIR NIR emitting QDs ( )O circulating lifetime ( ) . Organic dyes (min,) QDs , RES (reticuloendothelial system) , (48-72h)

  • QDs-PSMA Ab a) :b) background (autofluorescent)c) QDs d)

  • ( ) QDs

  • Multicolor quantum dots for molecular diagnostics of cancer Andrew M Smith, Shivang Dane, Shuming Nie,Lawrence True and Xiahu Gao Expert Rev.231-234 (2006)In vivo cancer targeting and imaging with semiconductor quantum dots Xiaohu Gao1, Yuanyuan Cui2, Richard M Levenson3, Leland W K Chung2 &Shuming Nie1 Article Nature Biotechnology 22, 969 - 976 (2004) Application of nanotechnology to biotechnology Jennifer L West and Naomi J Halas Current Opinion in Biotechnology 215-217 (2000)Quantum-dot-target microbeads for multiplexed optical coding of biomolecules Mingyong Han, Xiaohu Gao, Jack Z Su and Shuming Nie Nature Publishing Group 631-635 (2001)Quantum Dots paper Magda-Batista Carver (2006)http://en.wikipedia.org/wiki/http://www.cancer.gov/http://www.nature.com/index.htmlGoing small for big advances NCI (National cancer institute) article (2004)

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