ОПТИМИЗАЦИЯ КОНДУКТОМЕТРИЧЕСКОГО ТРЕХЭНЗИМНОГО БИОСЕНСОРА ДЛЯ ОПРЕДЕЛЕНИЯ ИОНОВ ТЯЖЕЛЫХ МЕТАЛЛОВ

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  • 86

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    EAmail: alex_sold@yahoo.com

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    7. C . ., . ., A . . . // . 2008. . 24, 1. . 4150.

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    11. Amine A., Mohammadi H., Bourais I. et al.Enzyme inhibitionbased biosensors for foodsafety and environmental monitoring //Biosens. Bioelectr. 2006. V. 21. P. 14051423.

    12. . . : . .... .. : 03.00.20 / . , 1999. 35 .

    13. ., . . .: , 1987. 286 .

    14. . ., . ., . . . 47 // . . . . 2005. 1. . 1420.

    15. TranAMinh C., Pandev P. C., Kumaran S.Studies on acetylcholine sensor and its analytical application based on the inhibition ofcholinesterase // Biosens. Bioelectr. 1990. V. 5. P. 46471.

    16. Mohammadi H., Amine A., Cosnier S. et al.Mercuryenzyme inhibition assays with anamperometric sucrose biosensor based on atrienzymaticclay matrix // Anal. Chim.Acta. 2005. V. 543. P. 143149.

    17. Bertocchi P., Ciranni E., Compagnone D. et al.Flow injection analysis of mercuriy(II) inpharmaceuticals based on enzyme inhibitionand biosensor detection // J. Pharm. Biomed.Anal. 1999. V. 20. P. 263269.

    18. . ., . ., A . . . // . 1994. T. 30, 8. . 982987.

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    21. Zhylyak G. A., Dzyadevich S. V., Korpan Y. I.et al. Application of urease conductometricbiosensor for heavymetal ion determination

    // Sens. Actuators B. 1995. V.24. . 145148.

    22. Volotovsky V., Kim N. EDTA determinationby ureasebased inhibition biosensor //Electroanalysis. 1998. V. 10, N1. P. 6163.

    23. Saran L., Cavalheiro E., Neves E. A. Newaspects of the reaction of silver (I) cationswith the ethylenediaminetetraacetate ion //Talanta. 1995. V. 42. P. 20272032.

    7

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    . , . (, , ), . . 1,25 , 1020 . Hg2+

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    OPTIMISATION OF CONDUCTOMETRICTHREENZYMES BIOSENSOR

    FOR HEAVY METAL DETECTION

    I. S. Kucherenko1, 2

    . . Soldatkin1

    V. M. Peshkova1, 2

    S. V. Dzyadevych1

    O. P. Soldatkin1

    1Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine,

    Kyiv2Taras Shevchenko National University of Kyiv

    EAmail: alex_sold@yahoo.com

    Data of conductometric biosensor optimization work for heavy metal ions determining inthe real samples are presented. A differential pairof the planar gold electrodes deposited on thepyroceramic substrate was used as a conductometric transducer. Threeenzymes system (invertase, mutarotase, glucoseoxidase) immobilizedon a transducers surface was used as a bioselective element. Developed biosensor demonstrateda good reproducibility of signal. Optimal concentration of sucrose for inhibition analysis was1.25 mM and incubation time in investigatedsolution amounted to 1020 min. Developedbiosensor demonstrated the best sensitivitytowards ions Hg2+ Ag+. Principal capability ofbiosensor reactivation by EDTA solution afterinhibition by silver ions or cysteine solutionafter inhibition by mercury ions was shown.Results of biosensor analysis of toxicants in realwater samples have a positive correlation withthe results obtained by traditional methods.

    Key words: biosensor, heavy metals, inhibitoryanalysis, enzymes, conductometric transducer.

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