Complete Proposal

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1 (230-444)

1.

1.1 :

1.2 : Study of the Constituents of Agarwood Smoke

2. : Chemical Engineering 3. 5010110186 5010110700 1.

2.

4. .. 5.

6.

2 ()

1,800

50 100

3 Sesquiterpene alcohol[6]

[Resin] Dihydroagarofuran, -Agarofuran, Agarofuran,Agarospirol Agarol[1]

7.

1. 2.

3. 8.

1.

9.

2.

9.1

[1]

18-21 1.5-1.8

4 5-10

(Resin) Sesquiterpene alcohol Dihydroagarofuran, b.Agarofuran, aAgarofuran, Agarospirol Agarol

2 1. (Volatile Organic Compounds, VOCs) 2. (Particulate Matter) VOCs)[2]

Lee and Wang (2004)

9.2 (Volatile Organic Compounds,

, , , , , , ,

VOCs , ,

5 VOCs 2 1.Non-chlorinated VOCs Non-halognated

hydrocarbons (gasoline)

aliphatic hydrocarbons ( fuel oils, , hexane, industrial solvents, aldehydes, ketone, hexane) ethylbenzene, xylenes, styrene, phenol)

aromatic hydrocarbons ( toluene, benzene, 2. Chlorinated VOCs halogenated hydrocarbons

Chlorinated

VOCs (Non-chlorinated VOCs)

9.3 (Particulate Matter)[3]

chloroform, vinyl chloride, dichloromethane

100

6

2 PM10 PM2.5 PM10 US. EPA

(Coarse Particle) 2.5 - 10

PM2.5 US. EPA

(Fine Particles) 2.5

VOC

PM10

Asthma PM2.5

7 9.4 Microextraction) SPME (Solid Phase[4]

Adsorption / Desorption Solvent Volatile Nonvolatile Headspace

(Volatile Organic Compounds) sensitivity for dilute analysts adsorb Volatile Compounds SPME poor

SPME polymer coated silica fiber fiber absorb injector

port GC GC-MS Desorb

GC GC-MS polymer coated fiber 1.1 1.2

1 SPME

8

1.2 SPME 9.4.1 SPME[4]

1.2 SPME

9.4.1.1 SPME

1 Stainless Steel Plunger Holder

SPME Fuse Silica Fiber Polymer

microlitresyringe Plunger Fiber 9.4.1.2 SPME

Headspace (Analyte) (Adsorption) polymer fiber

Fiber Polymer

Polymer

n = Coating

Co = Analyte

9 Kfs = Anayte Coate Vf = Coating Vs = ( 2-30 )

Sample Matrix

Fiber Vial Inject Injector GC Analyte Fiber Fiber HPLC Interface 2 Desorp GC Column 9.4.1.3 Fiber 1. Adsorbent Type Fiber Fiber

( High surface area )

analyte ( Porous Material ) , 2. Absorbent Type Fiber Fiber

( Partition ) like dissolved like ( Liquid Phase ) Fiber polymer silica fiber Volatiles

- 100 m polydimethylsiloxane ( 100 um PDMS) - 7 m polydimethylsiloxane Nonpolar High Molecular Weight Compounds

10 - 85 m polyacrylate Polarsemivolatiles -70 m Carbowax/divinylbenzene Alcohols and Polar Compounds Odor Compounds -50/30 m divinylbenzene/Carboxen

9.5 - (Gas Chromatography-Mass Spectrometry)[5]

Fingerprint (Mass number)

Library (Quantitative analysis) (Qualitative analysis) GC-MS 2 GC (Gas chromatography) Mass spectrometer 9.5.1. Gas Chromatography (GC)[5]

(Volatile organic

compounds) 2 Stationary phase Mobile phase

GC

11 3 1. Injector 2.Oven 3.Detector 2.1 9.5.2. Mass Spectrometer (MS)[5]

GC (Mass number) MS 1.) Ionization Source 2.) Mass Analyzer 2.2

Detector

2

2.2 Mass Spectrometer (MS) 10.

2.1 Gas Chromatography (GC)

12 Ishihara [6]

Aquilaria agallocha Roxb. Kanankoh and Jinko O

Kanankoh 100 gram Jinko 180-210 C 20 Diethyl ether GC-MS 30 mL/min Absorbent Tenax TA

Kanankoh Acetic acid ,Benzaldehyde Vanillin Aromatic Jinkoh (sesquiterpene) Jinkoh -Agarofuran, -Agarofuran, Agarospirol , Agarol Jinkohol [7]

/ SPME 4

-

DVB/CAR/PDMS, CW/DVB, PDMS/DVB CAR/PDMS DVB/CAR/PDMS PDMS/DVB CW/DVB CAR/PDMS 20

13 113 42 26 14 11 9 6 3 -Pinene, -Myrcene, Cymene, Limonene, Linalool, Benzyl acetate, Linalyl acetate Lilial

2 -Pinene,

DVB/CAR/PDMS PDMS/DVB CAR/PDMS CW/DVB PDMS/DVB DVB/CAR/PDMS [8]

CW/DVB CAR/PDMS

HS-SPME/GC-MS /

4 CW/DVB DVB/CAR/PDMS PDMS/DVB 15 o

PDMS PDMS/DVB

200 C linalool L (70.3 %) 1H-indole (17.5 %) linalool L (47.0%) benzyl acetate (24.4 %)

14 benzaldehyde (53.6 %) benzene acetaldehyde (13.8

%) -cubebene (10.3 %)

Spikenard benzylacetone -gurjunene (+)-calarene Spiknard Haxane benzylacetone ,-

Takemoto

[9]

calarene gurjunene calarene Spikenard benzylacetone , -gurjunene calarene benzylacetone 0.1% calarene 0.17% gurjunene 1.5% Flow Reactor Experiment Alfalfa Dalai [10]

15 o

60, 150 250 C 3 5, 20 45 GC-MS

48 Alfalfa

Alfalfa Alfalfa 1-octen-3-ol, (E)-3hexen-1-ol and (E)-2-hexanal Stem Solid Phase Microextraction Tran [11]

(SPME) Headspace (H/S) GCxGC GCxGC Low-

polarity/polar polar/non-polar

16 PAH, N-heterocyclics furans H/S SPME 65 m polydimethylsiloxane/divinylbenzene (PDMS/DVB)

324

100 200 Mass Spectroscopy (MS) 90

NIST library (National Institute of Standards and Technology) GCxGC 100 GCxGC [12]

GC 1

Headspace Solid Phase Microextraction (HSSPME) 2 polyacrylate (PA) divinylbenzene/carboxen/polydimethylsiloxane

(DVB/CAR/PDMS) - (GC-MS) 8 methyl butyrate, benzeneethanol, dihydro-5-pentyl-2(3H) benzaldehyde,2-amylfuran, benzenemethanol, isoamyl-2furanone, ethyl dodecanoate ethyl hexadecanoate

17 3 benzaldehyde, 2-amylfuran benzenemethanol 11. Aquilaria

crassna

GC-MS SPME

12. 1.

Thermocouple

Flow Reactor Experiment Tube furnace 3[10]

2.

5

Gas regulator 30 mL/min

18 100 % 60 150 250 Co

Mass flow meter (Sampling Bag) - (GC-MS) HP 5890 Gas Chromatograph-HP 5972 Mass Selective Detector

3 Flow Reactor Experiment [10]

3. SPME

PDMS/DVB fused silica fiber

PDMS/DVB polymer

H/S-SPME 4 Heater o

60 150 250 C

19 Heater Glass polymer 30

funnel Fused Silica Fiber Fiber GC-

MS HP 5890 Gas Chromatograph-HP 5972 Mass Selective Detector Structure NIST library (National Institute of Standards and technology) 4 [11]

4 HS-SPME Collects volatiles 13.

[11]

. .

/

. 20 . . . . 52 . . . 5 3 . . . . . .

52 52 1.

53 53

.

53 53 53

.

. 53

2. Flow Reactor 3. 4.

Flow Reactor MS GC-

5. polymer fused silica fiber H/S-

21

1 2.

Flow reactor experiment HS-SPME

() 4,000 10,000

Fused Silica Fiber MS GC

3.

3,000 17,000

15. [1]

Available online: http://www.ton[2]

. 2532. . kla.com/node/59, 14 2552.

2544. . Available online: 2552.

.

http://monitor.onep.go.th/document/voc.htm, 22 [3]

. 2552.

22 (Particulate Matter). Available online: pollution_pm.htm, 22 2552.

http://www.aqnis.pcd.go.th /basic/ [4]

Microextraction (SPME) : [5]

. Solid Phase E-mail:supat@smchem.co.th, :021961013

.

GC/MS. Available online: http://www.kmitl.ac.th/sisc/GCMS/main.html, 22 2552. , - -: : E-mail:

[7]

. /

winyu.chi@kmutt.ac.th [8]

Winyu Chitsamphandhvej. 2005. Qualitative Analysis of Volatile compositions of Dokputhachad (Jasminum Auriculatum) by using HS-SPME/GC-MS. 31st Congress 2005.

on Science and Technology of Thailand. 18 20 October

23 [9] Hiroaki Takemoto , Michiho Ito Tomohiro ,Shiraki , Toru Sedative effects of vapor inhalation of agarwood oil and spikenard extract and identification of their active components: Department of Pharmacognosy, Graduate 2006.

Yagura and Gisho Honda. 2006.

School of Pharmaceutical Science, Kyoto University, Japan. [10] Ajay Dalai, Greg Schoenau, Dharani Das and Phani Adapa. Volatile Organic Compounds emitted during High94 (1), 5766. temperature Alfalfa Drying. Biosystems Engineering. 2006: [11] C. Tran and P. J. Marriott. Characterization of incense

smoke by solid phase microextraction-Comprehensive twoEnvironment. 2007: 41 5756-5768.

dimensional gas chromatography (GC GC). Atmospheric [12] Sombat Nopichai, Yuthapong Udnan, Prinya Masawat

and Surat Boonphong. 2008.Volatile 2008: 5(2): 240 247.

compounds from Russula delica Fr. NU Science Journal.

24

16.

80-90

50 3

. 3

25 40 25 63

4 40 2 3 : . : Chemico biological/interactions

.. 2551

(Particulate Matter)

26 (Volatile Organic Compounds, VO