Genome Project

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Genome Project. . . . (budding yeast, S. cerevisiae): . 10_06_budding.yeast.S.jpg. 10_07_1_enzym.dideoxy.jpg. - PowerPoint PPT Presentation

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  • Genome Project .

    .

    .

  • 10_06_budding.yeast.S.jpg (budding yeast, S. cerevisiae):

  • 10_07_1_enzym.dideoxy.jpg

  • 10_07_2_enzym.dideoxy.jpg

  • 10_08_DNA.sequencing .jpg

  • Lecture 8 : Biological manipulation of the cell and genesGene Cloning DNA Recombinant DNA Technology DNA Genetic EngineeringIn vitro cultureStem cell lineTransgenesis ( ) -cells -animals :

  • 10_01_experiment.DNA .jpg

  • 10_03_Cells_origin.jpg ( ) . 1) 2) .in vitro culture cell sorting (CD markers)

  • (cell line) in vitro :

  • Gene Cloning DNA Recombinant DNA Technology DNA Genetic EngineeringIn vitro cultureStem cell lineTransgenesis ( ) -cells -animals

  • Steps of Recombinant DNA Technology ( )gene cloning -cDNA library screen . -PCR cloning

    Construction of recombinant vector (plasmid? Cosmid? phagemid?)Bacterial Transformation

    Genetic Manipulation -mutagenesis -promoter fusion -reporter constructTransformed cells and animals

  • by Library Screeningby PCRby Complementation (shot gun)Gene CloningGenomic vs. cDNA library

  • cDNA library =A still shot of a busy cross section

  • 10_21_DNA ligase.jpg

  • Why construct a recombinantplasmid?stablepropagation in bacteriaseparationWhy transform bacteria?stablestorageamplification

  • 10_22_cloned.DNA.frag.jpg

  • 10_24_hybridization.jpgColony Hybridization

  • Gene Cloning by Library ScreeningGene Cloning by PCRGene Cloning by Complementation

  • 10_27_1_PCR_amplify.jpg

  • 10_27_2_PCR_amplify.jpg

  • 10_28_PCR_clones.jpg

  • 10_29_PCR_viral.jpgPCR AIDS test

  • Recombinant DNA Technology1) DNA ( , , etc.)

    2)

    2) ( , )

    3) (in situ hybridization, reporter gene)

    4) ( , , )

    5)

  • Techniques in Genetic Engineering

  • 10_04_Restrict.nuclease.jpg1) Cutting and Joining DNA fragment

  • 10_18_ DNA.in.vitro.jpg

  • 10_05_gel.electrophor.jpg2) Hybridization ()

  • 10_12_de_renaturation.jpg

  • 10_13_hybridization.jpg

  • 10_14_1_Southrn.blotting.jpg

  • 10_14_2_Southrn.blotting.jpg

  • 10_16_In.situ.hybrdztn.jpg3) In situ hybridization ( )

  • 10_17_mRNA.in.situ.jpg

  • 10_15_DNA.microarrays .jpg4) Microarray

  • 10_19_DNA.uptake.jpg

  • 10_20_Bacteria.plasmid.jpg

  • 10_31_SerialDNA.clone.jpg

  • 10_32_expressionvector.jpg

  • 10_33_gene_protein.jpg

  • 10_34_Reporter.genes.jpg

  • 10_35_GFP.jpg

  • 10_36_mutagenesis.jpg

  • 10_37_engineered.org.jpg

  • 10_38_ES.cells.jpg

  • 10_39_Transgenic.mice.jpg

  • 10_40_Transgenic.plant.jpg

    10_06_budding.yeast.S.jpg10_07_1_enzym.dideoxy.jpg10_07_2_enzym.dideoxy.jpg10_08_DNA.sequencing .jpg10_01_experiment.DNA .jpg10_03_Cells_origin.jpg10_02_cell_sorter.jpg10_25_cDNA.jpg10_21_DNA ligase.jpg10_23_genomic.library.jpg10_22_cloned.DNA.frag.jpg10_24_hybridization.jpg10_27_1_PCR_amplify.jpg10_27_2_PCR_amplify.jpg10_28_PCR_clones.jpg10_29_PCR_viral.jpg10_04_Restrict.nuclease.jpg10_18_ DNA.in.vitro.jpg10_05_gel.electrophor.jpg10_12_de_renaturation.jpg10_13_hybridization.jpg10_14_1_Southrn.blotting.jpg10_14_2_Southrn.blotting.jpg10_16_In.situ.hybrdztn.jpg10_17_mRNA.in.situ.jpg10_15_DNA.microarrays .jpg10_19_DNA.uptake.jpg10_20_Bacteria.plasmid.jpg10_31_SerialDNA.clone.jpg10_32_expressionvector.jpg10_33_gene_protein.jpg10_34_Reporter.genes.jpg10_35_GFP.jpg10_36_mutagenesis.jpg10_37_engineered.org.jpg10_38_ES.cells.jpg10_39_Transgenic.mice.jpg10_40_Transgenic.plant.jpg