HPLC - ?· HPLC HPLC Specialist ... HPLC COLUMNS Table1. HPLC phase specifications. ... chemco-column英文カタログ.ppt…

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<ul><li><p>HPLCHPLC Specialist</p><p> Chemco Scientific Co., Ltd.</p><p>HPLC COLUMNS ChemcosorbChemcobondQUICKSORB</p><p>&amp;Application Data</p><p>URL ; http://www.chemco.jp/E-Mail ; info@chemco.jp</p><p>mailto:info@chemco.jphttp://www.chemco.jphttp://www.chemco.jpmailto:info@chemco.jp</p></li><li><p>URL ; http://www.chemco.jp/!E-mail : info@chemco.jp</p><p>CHEMCOSORB U-seriesCHEMCOSORB U-series packings are prepared from special silica with extremely large surface area (6nm pore,</p><p>600 m2/g). With this property of base material and our modification process, CHEMCOSORB U-series results in</p><p>more retentive and unique phase than other bland column. Wide range of chemical phase, ODS, C8, Diphenyl,</p><p>TMS, CN, NH2 and Si are available.</p><p>CHEMCOSORB 5-ODS-UH has very high carbon content (30%), using high surface area base material and</p><p>special modification process. This unique ODS phase has high capacity and resolution, and is particularly</p><p>effective not only precise separation of oils, fats and low polarity samples but also highly polar water soluble</p><p>samples, which are difficult to discriminate with conventional ODS phase. It is suitable for isomeric separation of</p><p>organic compounds and gradient elution of modified amino acids. Comparison of hydrophobic retention for other</p><p>brand ODS phase is shown in Fig-1.</p><p>Column size : 4.6mmx150mm.Mobile phase : MeOH/ Water =50/50 (v/v)Flow : 1.0 mL/min, Temp. : 40, Detection : UV 254 nm.Sample :1. Uracil, 2. Caffein, 3. Phenol, 4. Methylbenzoate,5. Benzene, 6. N, N-dimethyl aniline, 7. Toluene,8. Phenylacetylacetone</p><p>!"#</p><p>#"$</p><p>%"&amp;</p><p>&amp;"'</p><p>((")</p><p>(*"&amp;</p><p>(+"*</p><p>(+"&amp;</p><p>($"+</p><p>(&amp;")</p><p>!$"'</p><p>'"' +"' ('"' (+"' *'"' *+"' !'"' !+"' #'"' #+"'</p><p>,-./01234($</p><p>,-./035234$</p><p>67849:;234($</p><p>45?=4;,;@AB;A:BC</p><p>,-./03D234($</p><p>,-./03E24($</p><p>45?=4;,;@AB;A:B5</p><p>,-./03C24($</p><p>45?=4;:;</p></li><li><p>URL ; http://www.chemco.jp/!E-mail : info@chemco.jp</p><p>CHEMCOSORB 5-ODS-UH, the best material for scale up.</p><p>The retentive property of CHEMCOSORB 5-ODS-UH gives advantage in preparative chromatography. As shownin Fig-2, extremely large capacity of CHEMCOSORB 5-ODS-UH enables to load more concentrated sample than</p><p>other ODS columns, while maintaining good resolution under over load condition. In preparative</p><p>chromatography, CHEMCOSORB 5-ODS-UH is the best alternative to reduce total production cost and achieve</p><p>efficient separation.</p><p>Mobile phase; 80% MeOH, Column Size ; 4.6 mm I.d. x 150 mm. Flow ; 1.0 mL/min,</p><p>UV 300nm (over load injection) , Sample ; 1. Ethyl paraben, 2. Butyl paraben.</p><p>Conventional ODS</p><p>(350 m2/g, 14%C)</p><p>1 g Injection</p><p>(Injection vol. 1 L)</p><p>"#$$ % # &amp;'()*+,)(-.</p><p>1</p><p>2</p><p>"#$$ % # &amp;'()*+,)(-.</p><p>25, mg Injection</p><p>(Injection vol. 100 L)</p><p>"#$$ % # &amp; / 0'()*+,)(-.</p><p>Chemcosorb 5ODS-UH</p><p>(600 m2/g, 30%C)</p><p>"#$$ % # &amp; / 0'()*+,)(-.</p><p>Fig-2. Comparison of peak shape under over load condition.</p><p>U-13!Barbiturates</p><p>CHEMCOSORB 5ODS-UH</p><p>4.6!X150mm</p><p>CH3OH/H2O(49:51)</p><p>1.0mL/min, 4012 UV</p><p>220nm</p><p>1.Barbital 2.Allobarbital</p><p>3.Aprobarbital</p><p>4.Butabarbital 5.butarbital</p><p>6.Hexobarbital</p><p>7.Pentobarbital</p><p>8.Secobarbital</p><p>U-26! Nucleotides</p><p>CHEMCOSORB 5C8-U</p><p>4.6!X250mm</p><p>0.1M NH4H2PO4(pH</p><p>3.7/H3PO4)</p><p>1.5mL/min, 4012 UV 254nm</p><p>1.ATP 2.ADP 3.IMP 4.AMP</p><p>5.Hypoxannthine 6.Inosine</p><p>U-25!Fullerenes</p><p>CHEMCOSORB 5ODS-UH</p><p>4.6!X150mm</p><p>C6H6CH3/CH3CN(60:40)</p><p>1.5mL/min, ambient, UV @ 285nm</p><p>1. Fullerene C60, 2. Fullerene C70</p><p>U-19 34567849:;?:@</p></li><li><p>URL ; http://www.chemco.jp/!E-mail : info@chemco.jp</p><p>CHEMCOBONDTMCHEMCOBOND series packings are prepared from ultra pure silica gel that is</p><p>obtained by sol-gel technology. Due to its narrow particle size distribution,</p><p>CHEMCOBOND series give lower column back pressure than CHEMCOSORB. Peakshape for basic and chelateing solute are improved because of excellent endcaping</p><p>and metal free silica gel. ( Chemical phase; ODS, C8, NH2 and OH2 (diol), Particle</p><p>diameter; 3.5 m, 5 m, Pore diameter; 10 nm )</p><p>CHEMCOBOND ODS-W is specially designed ODS phase for polar solute separationin aqueous mobile phase. By optimizing silica pore size and ODS coverage, this</p><p>phase allows to use highly aqueous mobile phase for separation of polar solutes with</p><p>good retention reproducibility.</p><p>0 5 10 15</p><p>Tryptic digest of bovine serum albumin.</p><p>CHEMCOBOND 5-ODS-W, 4.6!x150mm</p><p>2.0 mL min, 0% A-60%B in 15 min.</p><p>A: 0.1% TFA, B: 0.1% TFA in Acetonitrile</p><p>CHEMCOBOND 3.5-ODS-H</p><p>QUICKSORBOur new ODS phase, QUICKSORB (18 nm pore, 200 m2/g, 13% carbon), is</p><p>suitable for high throughput HPLC separation. It reduces the actual analysis time in</p><p>isocratic elution. In spite of shorter analysis time, the chromatographic performance</p><p>is still maintained. In addition, the ultimate end capping and pure silica gel enableexcellent peak shape for basic and/ or chelating samples.</p><p>Fig-1 shows the chromatogram of 8 test solutes including basic and chelating</p><p>compounds. QUICKSORB separates these compounds with shorter and excellent</p><p>peak shape.Quicksorb 3 um</p><p>-H</p><p>B-8!Nucleosides</p><p>CHEMCOBOND 5ODS-H!4.6!X250mm</p><p>(22.5mM KH2PO4+2.5mM Na2HPO4)</p><p>[pH5.91]/CH3OH(91:9)</p><p>1.0mL/min, 4012 UV 260nm</p><p>1.Cytosine 2.Cytidine 3.Guanine 4.Uridine</p><p>5.Thymine 6.Guanosine 7.Adenine 8.Thymidine</p><p>9.Adenosine</p><p>B-11!Antidepressant Drugs</p><p>B-6!Anticonvalsants</p></li><li><p>URL ; http://www.chemco.jp/!E-mail : info@chemco.jp</p><p>First LC Column</p><p>Conventional Column</p><p>I.D., 3.0, 4.0, 4,6, 6,0 mm</p><p>Semi-Micro Column</p><p>I.D., 1.0, ( 2.0 ), 2.1 mm</p><p>Semi-Prep Column</p><p>I.D., 7.5, 10.0 mm</p><p>Prep ColumnI.D., 20.0, 30.0 mm</p><p>HPLC COLUMNS</p><p>Table1. HPLC phase specifications.</p><p>Chemcopak HPLC Columns</p><p>We can pack any size of column.</p><p>(Diameter 1.0 mm - 30 mm, column length</p><p>10 500mm.)</p><p>In addition to conventional size column,</p><p>micro capillary columns (diameter 0.1,</p><p>0.32, 0.53mm) are available for specific</p><p>application. These columns are sold by</p><p>name of chemcopak e-series.</p><p>HORIKAWA BLDG.,</p><p>2-8-14,NAKAZAKI, KITA-KU, OSAKA, JAPAN</p><p>Tel: +81-6-6375-0395!Fax: +81-6-6372-0533</p><p>Particle Pore Surface Carbon Particle Pore Surface CarbonDiameter Size Area Content Diameter Size Area Contentm (nm) (m2/g) (%) m (nm) (m2/g) (%)</p><p>QUICKSORB 3, 5 18 200 13 Si-U 5, 10 ----ODS-H 3.5, 5 10 340 22 C8-U 5, 10 14ODS-W 5 13 300 15 ODS-UH 5, 10 30C8 5 10 340 ---- Dph-U 5 ----NH2 3.5, 5 10 340 ---- CN-U 5 ----OH(Diol) 5 10 340 ---- NH2-U 5 ----Si 5, 7 8 ---- SCX 7 8 450 ----C8 7 8 9 SAX 7 8 450 ----ODS-L 5, 7, 10 10 10 300-CM 7 30 100 ----ODS-H 5, 7, 10 10 20 300-DEAE 7 30 100 ----Dph 5, 7 8 7 300-Si 7 100 ----TMS 5, 7 8 5 300-C4 7 100 0.8NH2 5, 7 8 ---- 300-C8 7 100 2.5CN 7 8 ---- 300-ODS-L 7 100 4120-Si ---- 300-C18 5, 7 30 100 6C8 8 300-Dph 7 100 1.2ODS-L 9 300-CN 7 100 ----ODS-H 18 300-NH2 7 100 ----Dph 6 300-OH 7 100 ----TMS 4 I-Si ----CN ---- I-C18 10NH2 ----</p><p>250</p><p>Column</p><p>CHEMCOSORB </p><p>6 600CHEMCOSORB </p><p>12</p><p>450</p><p>200</p><p> 5, 7, 10 10</p><p>CHEMCOSORB </p><p>Column</p><p>CHEMCOBOND </p><p>3</p></li><li><p>Specialist</p><p>URL ; http://www.chemco.jp/E-Mail ; info@chemco.jp</p>mailto:info@chemco.jphttp://www.chemco.jphttp://www.chemco.jpmailto:info@chemco.jp</li></ul>