Ionenströme als

  • Published on
    31-Dec-2015

  • View
    29

  • Download
    3

Embed Size (px)

DESCRIPTION

Ionenstrme als. Krebsdetektive. Mglicher Ansatz fr eine Therapie ?. Isabella Derler Institut fr Biophysik. 2. hufigste Erkrankung in Europa und den USA nach den Herz-Kreislauf-Erkrankungen. Krebssterblichkeit 1992-2004 sterreich WHO-Standardbevlkerung, altersstandardisiert. - PowerPoint PPT Presentation

Transcript

<ul><li><p>Ionenstrme alsIsabella DerlerInstitut fr BiophysikMglicher Ansatz fr eine Therapie ?Krebsdetektive</p></li><li><p>Krebssterblichkeithttp://www.statistik.at/fachbereich_03/gesundheit_txt.shtmlProstatakrebs-gewebe</p></li><li><p>Krebsentstehung</p></li><li><p>Krebszellen sind verndertGesundes Prostata-gewebeProstata-KREBS-gewebeProtein, das nur in der Krebszelle vorkommt.Nur welches?</p></li><li><p>Das unbekannte Protein Ion (geladenes Atom)</p></li><li><p> ein IonenkanalTransient receptor potential (TRP) channelals Ursache fr Krebs ?</p></li><li><p>Was ist ein TRP - Protein?transient vorbergehend?nur welche Ionen Na+Ca2+Mg2+</p></li><li><p>Messung des IonenstromsI</p></li><li><p>Elektrode, die ins Zellinnere reichtZahl der Ionen, die durch einen Kanal gehen ~ 10 Mio (107) Ionen / s=&gt;Stromgre fr einen Ionenkanal: ~ 1 billionstel (10-12 od. pico) Ampere=&gt;Strom ber Ionenkanle in der ganzen Zelle: ~ 1 milliardstel (10-9 od. nano) AmperePatch-Clamp Technikhttp://www.science-display.com/patchclamp.html</p></li><li><p>Patch-Clamp Aufbau</p></li><li><p>Tter: Kalziumionen (kanal)Kampf dem Kalziumstrom !Ca2+ Ionen</p></li><li><p>Calmodulinaktivwie ein Regenschirm</p></li><li><p>Blocker: CalmodulinZeitStrom</p></li><li><p>Bindet CaM am Kalzium-Kanal ?Ermittelung ber FluoreszenzmikroskopieProteinFarbstoffFluoreszenz2 Proteine, die aneinander binden (Nhe: &lt; 10 milliardstel Meter (10 nm))Signal der Interaktion</p></li><li><p>Ja! Fluoreszenz als Aus-ma der InteraktionZeitCa2+ EinstromBindet CaM am Kalzium-Kanal ?1212</p></li><li><p>Blockierung des Kalziumstroms</p></li><li><p>Danke fr eure Aufmerksamkeit !</p></li><li><p>Prostatakrebsgewebe</p></li><li><p>Ursachen fr Krebs</p></li><li><p>Atom - IonProtonenNeutronenElektronenKern:Hlle:</p></li><li><p>Fluoreszenz</p><p>Cancer develops when cells in a part of the body begin to grow out of control. Normal cells grow, divide and die in an orderly way. Abnormal cells develop when DNA, the bodys genetic code, is damaged. Exposure to chemicals or radiation causes most DNA damage, but sometimes a mutation is inherited or caused by a virus. Instead of dying, cancer cells continue to form new abnormal cells. Scientist think that, over time, many mutations occur through the cancer stages of initiation, promotion and progression (see figure). Eventually, abnormal cells form a tumor and may spread to other parts of the body. Cancer develops when cells in a part of the body begin to grow out of control. Normal cells grow, divide and die in an orderly way. Abnormal cells develop when DNA, the bodys genetic code, is damaged. Exposure to chemicals or radiation causes most DNA damage, but sometimes a mutation is inherited or caused by a virus. Instead of dying, cancer cells continue to form new abnormal cells. Scientist think that, over time, many mutations occur through the cancer stages of initiation, promotion and progression (see figure). Eventually, abnormal cells form a tumor and may spread to other parts of the body. Cancer develops when cells in a part of the body begin to grow out of control. Normal cells grow, divide and die in an orderly way. Abnormal cells develop when DNA, the bodys genetic code, is damaged. Exposure to chemicals or radiation causes most DNA damage, but sometimes a mutation is inherited or caused by a virus. Instead of dying, cancer cells continue to form new abnormal cells. Scientist think that, over time, many mutations occur through the cancer stages of initiation, promotion and progression (see figure). Eventually, abnormal cells form a tumor and may spread to other parts of the body. </p></li></ul>