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Lab meeting. 2014.02.24 이세라. Gene Scan –B2M. PCR. electrophoresis. Sample 2. Sample 1. 95℃ / 5min. Sequencing-PLK1. Childhood AML -RNA. cDNA 합성. 65 ℃ / 5min -> ice. 2 5 ℃ / 10min -> 50 ℃ / 50min ->85 ℃ / 5min -> ice. 1 μ l of Rnase H. 37 ℃ / 20min -> ice. cDNA ->electrophoresis. - PowerPoint PPT Presentation

Text of Lab meeting

Lab meeting

Lab meeting

2014.02.24Gene Scan B2MSample nameSample 7095224211/09CRS7095184911/16CRS

PCR

Sample 2.

Sample 1.electrophoresis95 / 5min

Sequencing-PLK1

Childhood AML -RNA

cDNA

65 / 5min -> ice

25 / 10min -> 50 / 50min->85 / 5min -> ice1l of Rnase H37 / 20min -> icecDNA->electrophoresis

cDNA PCR->electrophoresisPLK1 -Primer

pyrosequencing

A-365B-2009-129D-4D-222014.02.24(BDNF)PCRPCR

2013.09.24(SLC6A4)flowcytomety2014.02.21

G-MDSCM-MDSC

G-MDSCM-MDSC2/20sample 71411924