Lab meeting

  • Published on
    17-Jan-2016

  • View
    19

  • Download
    0

Embed Size (px)

DESCRIPTION

Lab meeting. 2014.02.24 . Gene Scan B2M. PCR. electrophoresis. Sample 2. Sample 1. 95 / 5min. Sequencing-PLK1. Childhood AML -RNA. cDNA . 65 / 5min -> ice. 2 5 / 10min -> 50 / 50min ->85 / 5min -> ice. 1 l of Rnase H. 37 / 20min -> ice. cDNA ->electrophoresis. - PowerPoint PPT Presentation

Transcript

Lab meeting

Lab meeting

2014.02.24Gene Scan B2MSample nameSample 7095224211/09CRS7095184911/16CRS

PCR

Sample 2.

Sample 1.electrophoresis95 / 5min

Sequencing-PLK1

Childhood AML -RNA

cDNA

65 / 5min -> ice

25 / 10min -> 50 / 50min->85 / 5min -> ice1l of Rnase H37 / 20min -> icecDNA->electrophoresis

cDNA PCR->electrophoresisPLK1 -Primer

pyrosequencing

A-365B-2009-129D-4D-222014.02.24(BDNF)PCRPCR

2013.09.24(SLC6A4)flowcytomety2014.02.21

G-MDSCM-MDSC

G-MDSCM-MDSC2/20sample 71411924