PENGOPTIMUMAN PEWARNAAN IMUNOHISTOKIMIA etd. ?· Gambar 3.1. Lokasi biopsi kulit kaki mencit ... LPPT…

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vi

DAFTAR ISI

HALAMAN JUDUL ............................................................................................ .. i

HALAMAN PENGESAHAN ............................................................................... . ii

HALAMAN PERNYATAAN ............................................................................. iii

PRAKATA ........................................................................................................... iv

DAFTAR ISI ........................................................................................................ vi

DAFTAR GAMBAR ........................................................................................... ix

DAFTAR TABEL ................................................................................................ . x

DAFTAR LAMPIRAN ........................................................................................ xi

ARTI LAMBANG DAN SINGKATAN ............................................................. xii

INTISARI ............................................................................................................ xiii

ABSTRACT ........................................................................................................ xiv

BAB I PENDAHULUAN .................................................................................... . 1

I.1. Latar Belakang ..................................................................................... . 1

I.2. Perumusan Masalah .............................................................................. . 3

I.3. Tujuan Penelitian ................................................................................... . 3

I.4. Keaslian Penelitian ............................................................................... . 3

I.5. Manfaat Penelitian ................................................................................ . 4

BAB II TINJAUAN PUSTAKA ......................................................................... . 5

II.1. Tinjauan Pustaka ................................................................................. . 5

II.1.A. Serabut Saraf Intraepidermal ................................................. . 5

II.1.B. Imunohistokimia .................................................................... . 8

vii

II.1.C. Perbandingan Metode Pewarnaan Imunohistokimia Dengan

Antibodi Anti PGP 9.5 ...................................................................... 11

II.2. Landasan Teori .................................................................................... 16

II.3. Kerangka Teori ................................................................................... 17

II.4. Kerangka Konsep ................................................................................ 18

BAB III METODE PENELITIAN ....................................................................... 19

III.1. Jenis dan Rancangan Penelitian .......................................................... 19

III.2. Variabel Penelitian ............................................................................. 19

III.3. Definisi Operasional ........................................................................... 19

III.4. Bahan dan Alat Penelitian ................................................................. 20

III.4.A. Subjek Penelitian .................................................................. 20

III.4.B. Bahan Penelitian ................................................................... 20

III.4.C. Alat Penelitian ...................................................................... 20

III.5. Jalannya Penelitian ............................................................................. 22

III.6. Analisis Hasil Penelitian .................................................................... 28

BAB IV HASIL DAN PEMBAHASAN ............................................................. 29

IV. 1. Hasil Penelitian ................................................................................. 29

Pengoptimuman Pewarnaan Imunohistokimia Dengan Antibodi Anti PGP

9.5 ................................................................................................................ 29

IV.2. Pembahasan ....................................................................................... 34

BAB V KESIMPULAN, SARAN DAN RINGKASAN ..................................... 37

V.1. Kesimpulan ......................................................................................... 37

V.2. Saran ................................................................................................... 37

viii

V.3. Ringkasan ........................................................................................... 38

V.3.A. Latar Belakang........................................................................ 38

V.3.B. Landasan Teori ...................................................................... 38

V.3.C. Cara Penelitian ........................................................................ 39

V.3.D. Hasil ........................................................................................ 40

V.3.D. Kesimpulan ............................................................................ 40

DAFTAR PUSTAKA .......................................................................................... 41

LAMPIRAN .......................................................................................................... 50

ix

DAFTAR GAMBAR

Gambar 2.1. Struktur lapisan kulit dan reseptor-reseptor sensoris yang terlibat

dalam sensasi sentuhan ...................................................................... . 6

Gambar 2.2. Lokasi inervasi dan penjuluran serabut saraf intraepidermal kornu

dorsalis ............................................................................................. . 7

Gambar 2.3. Pewarnaan imunohistokimia secara langsung (direk) ..................... . 9

Gambar 2.4. Pewarnaan imunohistokimia secara tidak langsung (indirek) ......... 10

Gambar 2.5. Kerangka teoritis deteksi serabut saraf intraepidermal dengan metode

imunohistokimia ................................................................................. 17

Gambar 2.6. Kerangka konsep penelitian pengoptimuman pewarnaan

imunohistokimia dengan antibodi anti PGP 9.5 untuk deteksi serabut

saraf intraepidermal dari biopsi kulit ................................................ 18

Gambar 3.1. Lokasi biopsi kulit kaki mencit ....................................................... 24

Gambar 3.2. Aturan penghitungan serabut saraf intraepidermal ......................... 28

Gambar 4.1. Hasil Pengoptimuman Tahap I ........................................................ 30

Gambar 4.2. Gambaran serabut saraf intraepidermal dengan antibodi anti PGP 9.5

........................................................................................................ 31

x

DAFTAR TABEL

Tabel 2.1. Perbandingan Metode Pewarnaan Imunohistokimia Dengan Antibodi

Anti PGP 9.5 ..................................................................................... 13

Tabel 4.1 Hasil Pengoptimuman Pewarnaan Imunohistokimia Dengan Antibodi

Anti PGP 9.5 .................................................................................... 33

xi

DAFTAR LAMPIRAN

Ethical Clearance.................................................................................................. 50

xii

ARTI LAMBANG DAN SINGKATAN

BB Berat Badan

DAB Diaminobenzidine

EFNS European Federation of Neurological Societies

HRP Horseradish peroxidase

LPPT Lembaga Pengujian dan Penelitian Terpadu

NA Numerical aperture

PBS Phosphate Buffered Saline

PGP 9.5 Protein gene product 9.5

UPHP Unit Pengembangan Hewan Percobaan

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