PeproTech Product Update January 2011

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The UPDATE is a newsletter published on a quarterly basis.

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  • JANUARY 2011

    Continued on next page

    MANUFACTURER OF QUALITY CYTOKINE PRODUCTS W W W . P E P R O T E C H . C O M

    BMP-4 (E.coli) fully active dimer(Catalog #120-05ET)

    Synonyms: BMP-2B, DVR4Description: Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF- superfamily of structurally relat-ed signaling proteins. Members of this superfamily are widely distributed throughout the body and are involved in diverse physiological processes during both pre- and postnatal life. Like BMP-7, BMP-4 is involved in the development and main-tenance of bone and cartilage. Reduced expression of BMP-4 is associated with a number of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Pep-roTechs E. coli derived BMP-4 is a fully active homodimeric protein consisting of two 106 amino acid subunits, which cor-respond to amino acids 303 408 of the full length BMP-4 precursor. E.coli derived BMP-4 represents a readily available and affordable alternative to cell culture derived material. Cell culture derived BMP-4 is also available, Catalog #120-05.

    CDNF (Catalog #450-05)Synonyms: Cerebral dopamine neurotrophic factor, ARMETL1Description: CDNF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. It has been shown to promote survival, growth and function of dopamine specific neurons. CDNF and its structural ho-

    NEW - PeproTech Publications Now available for order and pre-order (see inside mailer flap for details)

    molog MANF, each contain an N-terminal saposin-like lipid binding domain, and a carboxyl-terminal domain, which is not homologous to previously characterized protein struc-tures. CDNF and MANF can prevent 6-OHDA induced de-generation of dopaminergic neurons by triggering survival

    0.30

    0.60

    0.90

    1.20

    1.50

    1.80

    0.0 0.1 1.0 10.0 100.0

    OD

    (405 n

    m)

    h-BMP-4 (ng/ml) [log scale]

    BioAssay for Human BMP-4 using ATDC-5 Cell Line

    Human BMP-4; PeproTech cell culture BMP-4 (catalog #120-05)

    Human BMP-4; competitor, cell culture derived

    Human BMP-4; PeproTech E.coli derived BMP-4 (catalog #120-05ET)

    2011 Weekly Planner Cytokine INDEX (3rd edition) 2011-2012 PeproTech Catalog 2011 PeproTech Price List 2011-2012 USB Catalog

  • CDNF Continued from page 1

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    pathways in a rat experimental model of Parkinson disease. Recombinant human CDNF is an 18.5 kDa protein consisting of 162 amino acids including 8 cysteine residues.

    sCD27L (Catalog #310-30)Synonyms: TNFSF7, CD70Description: CD27 Ligand, a type II transmembrane protein, is a member of the TNF superfamily. It is expressed on activated T and B lymphocytes as well as NK cells. CD27L and its receptor (CD27) regulate the immune response by promoting T-cell ex-pansion and differentiation, as well as NK enhancement. CD27 signaling can act as a co-stimulatory effector to sustain the sur-vival of CD8+ T cells, primarily by inducing increased expres-sion of the IL-2 gene. Full length human CD27L is a 193 amino acid protein, consisting of a 17 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 155 amino acid extracellular domain. Human soluble CD27L corresponds to the 155 amino acid extracellular domain of the full length CD27L protein. PeproTechs recombinant human sCD27L contains the extracellular domain plus an N-terminal His-Tag.

    Chemerin (Catalog #300-66)Synonyms: Tazarotene-induced gene 2 (TIG2), RARRES2Description: Chemerin is a secreted chemoattractant protein that can signal through the chemokine like receptor-1 (CM-KLR1). It is expressed in various tissues, including white adi-pose tissue, and circulates in blood as an inactive 143 amino acid precursor protein. Biologically active Chemerin is generated by proteolytic removal of C-terminal residues by several circulating proteases. Chemerin acts as a chemoattractant for cells express-ing the CMKLR1 receptor, which includes certain dendritic cells, macrophages, and adipocytes. Recombinant human Chemerin is a 15.6 kDa protein consisting of 135 amino acid residues.

    E-Selectin (Catalog #150-15)Synonyms: Endothelial leukocyte adhesion molecule 1

    (ELAM1), CD62E, SELEDescription: Selectins are a family of calcium-dependent type 1 transmembrane proteins. Endothelial (E)-selectin is a heavily glycosylated transmembrane protein expressed by activated en-dothelial cells in microvascular linings. E-selectin, along with P-selectin and L-selectin, initiate recruitment of circulating leu-kocytes from blood to sites of inflammation in the vascular lining through interaction with specific cell surface associated carbohy-drate determinants. E-selectin consists of an N-terminal type 1 lectin domain, an EGF-like domain, 6 sushi (CCP/SCR) domains,

    a transmembrane sequence, and a short cytoplasmic domain. Re-combinant human E-selectin is a 58.6 kDa protein containing 535 amino acid residues, corresponding to the extracellular portion of the full length protein. Due to glycosylation, E-selectin migrates at an apparent molecular weight of approximately 65-85 kDa by SDS-PAGE analysis under reducing conditions.

    Human IL-34 (Catalog #200-34)Synonyms: C16orf77Description: IL-34 is a homodimeric cytokine that is ex-pressed in a range of tissues including spleen, heart, brain, liver, kidney, lung, ovary, thymus, testis, small intestine, prostate and colon. IL-34 is a ligand for colony-stimulating factor-1 recep-tor (CSF1R), which also binds to CSF-1. It specifically binds CD14+ monocytes, promotes survival and proliferation of hu-man peripheral blood monocytes, and stimulates macrophage colony formation by human bone marrow cells. IL-34, like hu-man CSF-1 stimulates phosphorylation of MAPK1/ERK2 and MAPK3/ERK1. Recombinant human IL-34 is a 52.5 kDa ho-modimeric glycoprotein consisting of 460 amino acid residues, including a C-terminal His-Tag.

    MMP-3 (Catalog #420-03)Synonyms: Stromelysin-1, SL-1, Transin-1 Description: Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing cata-lytic activity. These enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovas-cular disease, skeletal growth-plate disorders, and cancer metas-tasis. MMP-3 degrades fibronectin, laminin, collagens III, IV, and X, and cartilage proteoglycans. Recombinant human MMP-3 is a 42.8 kDa protein containing 378 amino acids, which correspond to residues 100 477 of the full length MMP-3 precursor protein.

    SPARC/Osteonectin (Catalog #120-36)Synonyms: Secreted protein acidic and rich in cysteine,

    BM-40, ONDescription: SPARC/Osteonectin is a secreted, evolution-arily conserved collagen-binding glycoprotein that is involved in a variety of cellular activities. It is highly expressed in tis-sues undergoing morphogenesis, remodeling and wound repair. SPARC/Osteonectin and its related peptides bind to numerous proteins of the extracellular matrix (ECM), affect ECM protein expression, influence cellular adhesion and migration, and mod-ulate growth factor-induced cell proliferation and angiogenesis. SPARC/Osteonectin consists of three domains; an N-terminal acidic region that binds calcium ions with low affinity, a module containing two EF-hand motifs that bind calcium with high af-finity, and a cysteine-rich follistatin-like domain. Recombinant human SPARC/Osteonectin is a glycoprotein containing 286 amino acids that migrates at an apparent MW of 43.7 kDa by SDS-PAGE analysis due to the effect of glycosylation.

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    PeproTech is pleased to announce the release of its ELISA Buffer Kit. The buffers in this supplemental kit have been specially formulated for optimal perfor-mance when used in conjunction with PeproTechs ELISA Development Kits. The buffer component kit contains all of the necessary components to assay ten 96 well ELISA plates (included) and contains detailed handling instructions. All of the reagents have been sterile filtered to minimize assay interference and maximize shelf life. The ELISA Buffer Kit can be pur-chased along with PeproTechs ELISA Development Kit at a discounted price.

    This easy-to-use ELISA buffer kit can also be pur-chased as a stand-alone product since the plates included are not pre-coated with capture antibody. This format allows the researcher to develop and optimize an assay for use with their own capture and detection antibodies. The actual antibody concentra-tions and detection ranges of the ELISA will vary.

    PeproTechs New

    ELISA Buffer Kit

    Kit Contents: - 20X Plate Coating Buffer

    - 1X Blocking Buffer

    - 20X Sample Diluent

    - 20X Wash Buffer

    - Ready-to-use ABTS

    - 10 Sterile, Uncoated 96 well ELISA Plates

    - 50 Plate Sealing Films

    - Buffer Handling Instructions

    Monoclonal AntibodiesAnti-human Eotaxin ............................... 500-M25Anti-human IL-6 .................................... 500-M06

    ELISA Development KitsHuman BD-4 .........................................900-K435Murine IL-17 .........................................900-K392Human Leptin .........................................900-K90Human MIG ............................................900-K89Human TIMP-1 .....................................900-K438

    Mini - ELISA Development KitsHuman BD-1 .........................................900-M202Human BD-2 .........................................900-M172Human BD-4 .........................................900-M435Human sCD40L ....................................900-M145Human CXCL16 ...................................900-M230Human EGF ............................................900-M05 Human FGF-basic ...................................900-M08Human IFN-g ..........................................900-M27Murine IFN-g ..........................................900-M98Murine IL-1 ..........................................900-M47Human IL-2 .............................................900-M12Human IL-4 .............................................900-M14Human IL-5 .............................................900-M15Human IL-6 .............................................900-M16Rat IL-6 ...................................................900-M86Human IL-8 .............................................900-M18Murine IL-10 ...........................................900-M53Human IL-12 ...........................................900-M96Murine IL-12 ...........................................900-M97Murine IL-13 .........................................900-M207Human IL-17A ........................................900-M84Murine IL-17 .........................................900-M392Human IL-17E ......................................900-M234Human IL-31 .........................................900-M347Human IL-33 .........................................900-M398Murine IP-10 .........................................900-M153Murine JE/MCP-1 .................................900-M126Murine KC ............................................900-M127Human MIG ............................................900-M87Murine MIP-1 .....................................900-M278Human RANTES ....................................900-M33Human TNF- ........................................900-M25Murine TNF- ........................................900-M54Human VEGF .........................................900-M10

    ProteinsHuman BMP-3 .......................................120-24BMurine Betacellulin ................................. 315-21Human sCD30Ligand/TNFSF8 ............... 450-42Rat Carboxypeptidase-B .......................... 400-00Human Enterokinase ..............................450-48CHuman E-Selectin .................................... 150-15Human IFN-omega .................................300-02JRat IL-9 .................................................... 400-18Human IL-23 ............................................ 200-23Human Nanog-TAT ................................120-21BMycoplasma Arginine Deiminase ............ 150-12Rat Oncostatin M ..................................... 400-36Human p16INK4a-TAT .......................... 110-02THuman Oct4-TAT ...................................110-04H

    Antibodies -Polyclonal/BiotinylatedAnti-human Heregulin-1 ..................... 500-P288Biotinylated Anti-hHeregulin-1 ....... 500-P288btAnti-human IGF-BP1............................ 500-P287Biotinylated Anti-hIGF-BP1 .............. 500-P287btAnti-human Maspin .............................. 500-P270Biotinylated Anti-hMaspin................. 500-P270btAnti-human Oct4-TAT .......................... 500-P289Biotinylated Anti-hOct4-TAT ............ 500-P289btAnti-human rat IP-10 ............................ 500-P290Biotinylated Anti-rIP-10 .................... 500-P290bt

    More New Productsfrom PeproTech!

  • A PUBLICATION OF PEPROTECH INC. WWW.PEPROTECH.COM

    PRODUCT UPDATEThis publication is intended to update our customers about products

    and services offered by PeproTech.

    Published by PeproTech Inc.

    PeproTech Asia:12 Hamada Street, Tamar Building

    Rehovot 76703 IsraelTel: +972 8 946 0948. Fax: +972 8 946 0861

    info@peprotechasia.com www.peprotech.comwww.peprotech.com

    We welcome you to visit us at the following meetings in 2011

    - 4 -

    PeproTech Trade Shows 2011

    The Tradeshow Schedule above is tentative and is subject to change. To see our most up-to-date schedule, please visit our website, www.peprotech.com.

    * Dates undetermined at time of printing.

    Date Name of Show Location

    March 30 - April 1 UK National Stem Cell Network Meeting York, England

    April 2-6 American Association for Cancer Research Orlando, FL (AACR)

    April 9-13 Experimental Biology Washington, DC

    April 18-19 British Microcirculation Meeting London, England

    April 27-30 Joint Spring Meeting of the British Society of Canterbury, England Cell Biology & British Society of Developmental Biology (BSCB/BSDB)

    May 13-17 Immunology 2011 San Francisco, CA

    May 18-21 17th Annual Meeting of the International Rotterdam, Society for Cellular Therapy (ISCT) The Netherlands

    May 18-19 NIH Spring Research Festival Bethesda, MD

    May 25-27 13th Annual Meeting of the French Society Paris, France of Biology of Mineralised Tissues (SFBTM)

    May 25-27 Cancer Immunotherapy Meeting (CIMT) Mainz, Germany

    May 26-29 17th Symposium of Czech Reproductive Zdar nad Sazavou, Immunology Czech Republic

    May 29 - June 1 Canadian Neuroscience Meeting Quebec City, Canada

    May 30 - June 1 Colloque des Cytokines Le Croisic, France

    June 15-18 International Society for Stem Cell Research Ontario, Canada (ISSCR)

    June 25-29 10th World Congress on Inflammation Paris, France

    August* Universidad Nacional Autonoma de Mexico Mexico City, Mexico

    September* Universidad Autonoma Metropolitana Mexico City, Mexico

    September 11-14 Stem Cells in Develo...

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